San Francisco de Asis, popularly called Mission Dolores, has been in pretty much continuous use since it was dedicated on April 3, 1791. The mission declined after secularization and portions of the mission quadrangle were sold or leased to business firms. There was even a Tavern in one of the buildings. However even in those days the chapel was used for religious services.
By 1876 the city had grown a lot and the community needed a bigger Parish church, so a large Victorian-style church was built next door. This structure was severely damaged by the 1906 earthquake and had to be taken down.
Between 1913-1918 the large stone mission-style church you see today was built. However, during all these years up to today the original mission chapel has been used for special religious services.
The last time I was there a boy was being baptized in the original Mission Dolores chapel.